Fertilization affects soil nitrogen cycling and nitrous oxide (N2O) emissions, which are mainly driven by microbes. A 32-year field experiment was conducted to investigate the effects of chemical fertilizers and their combination with organic materials on the abundance of denitrifying functional genes (nirS, nirK, nosZ I and nosZ II) in Ultisol. The treatments comprised no fertilizer (CK), chemical fertilizer, chemical fertilizer+peanut straw, chemical fertilizer+rice straw, chemical fertilizer+radish and chemical fertilizer+pig manure. Compared with the single chemical fertilizer treatment, soil pH and organic carbon content increased in the chemical fertilizer plus organic material treatments, with chemical fertilizer+pig manure having the strongest effect. Long-term fertilization did not affect the abundance of nirK gene, but significantly altered the nirS gene abundance. Compared to CK, long-term chemical fertilizer application increased the abundance of nirS gene by 426%. However, partial replacement of chemical fertilizer by organic materials decreased the abundance of nirS gene. The abundance of nosZ I gene was one order of magnitude higher than that of nosZ II, indicating the domination of nosZ I in the acidic Ultisol. Long-term fertilization did not affect the abundance of nosZ II, whereas chemical fertilizer+pig manure increased the abundance of nosZ I by 138%. Results of stepwise regression analysis showed that available phosphorus content was the primary factor regulating the abundance of nosZ I gene, whereas the abundance of the nosZ II gene was mainly regulated by nitrate content. Moreover, the lowest (nirS+nirK)/(nosZ I+nosZ II) value in the chemical fertilizer+pig manure treatment indicated that long-term manure application might reduce N2O emission potential in Ultisols.A field experiment was conducted for two seasons to evaluate the application effects of a decision support system named Nutrient Expert on radish based on yield response and agronomic efficiency, to provide theoretical and technical support for convenient and quick recommendation on fertilization management. There were seven treatments farmer's practice treatment (FP), recommended fertilization treatment based on Nutrient Expert (TE), recommended fertilization treatment based on soil testing (TS), treatment of replacing 30% nitrogen fertilizer with organic fertilizer based on TE (TE+OM), and corresponding nitrogen omission treatment (TE-N), phosphorus omission treatment (TE-P), and potassium omission treatment (TE-K). We measured and compared the effects of different fertilization managements on radish yield, nutrient uptake, fertilizer utilization and fertilization benefit. The results showed that the N, P2O5 and K2O fertilizer applications based on Nutrient Expert were 200, 132 and 215 kg?hm-2 in the first could make full use of the indigenous nutrients of soil, consider the balance and sustainable supply, and reasonably regulate the supply of nitrogen, phosphorus and potassium, and finally result in high yield, high efficiency and sustainable development of radish production.To understand the effects of activated water irrigation on soybean growth under different drought conditions and explore the underlying mechanisms, an indoor pot experiment was conducted under four moisture conditions of 95%-100%, 75%-85%, 55%-65% and 35%-45% of the maximum water holding capacity (80% moisture) of the medium. Soybean was irrigated with tap water, magnetized water, aerated water, and magnetized and then aerated water, respectively. The results showed that total biomass, leaf area, root to shoot ratio, and root length of magnetized water irrigation increased by 67.6%, 23.5%, 84.6% and 122.8%, respectively compared with tap water irrigation after 30 days of growth under 35%-45% severe drought condition. All the variables were increased by 70.8%, 24.0%, 61.9% and 162.3% respectively in magnetized and aerated water treatment. There was no significant difference for foliar chlorophyll content. The values of the other water treatments were slightly lower than that of tap water. In conclusion, magnetized water irrigation effectively enhanced root growth, root-shoot ratio, and water use efficiency of soybean, and alleviated the negative effects of drought stress under severe drought condition.Under the same irrigation amount and nitrogen application rate and after the corn stalks being returned to the field in the wheat-corn crop rotation area, we examined the effects of the integrated water and nitrogen mode of micro-sprinkler irrigation on the growth and development and water and fertilizer use efficiency of winter wheat. In 2016-2018, we conducted a two-year field experiment with six types of micro-sprinkler irrigation water and nitrogen integration modes and seven treatments during the growth period, and investigated the population dynamics, dry matter accumulation transfer during the filling period, and nutrient accumulation during the mature period. There were three modes of irrigation, W1(overwintering water + jointing water + grouting water, 600 m3?hm-2 for each), W2(overwintering water + regreening water + jointing water + grouting water, each for 450 m3?hm-2), and W3(600 m3?hm-2 each for overwintering water and jointing water, and 300 m3?hm-2 each for regreening water and grouting water) and 1000-grain weight of wheat andincreased yield, with the highest water and fertilizer use efficiency. https://www.selleckchem.com/products/mrtx1133.html It was the best water and nitrogen management mode for the integration of micro-sprinkler irrigation and water and fertilizer for winter wheat in southern Shanxi.To promote the rational application of nitrogen fertilizer in winter wheat after rice stubble, the effects of nitrogen application rate (0, 150, 225, 300 kg?hm-2, expressed as N0, N150, N225, N300) on nitrogen recovery, residue, loss and grain yield were examined using field 15N tracer technology. The results showed that with the increases of application rate, nitrogen accumulation from different sources significantly increased while nitrogen recovery significantly decreased. The accumulation of basal nitrogen in plants reached the peak during overwintering stage to jointing stage, while the accumulation of topdressing nitrogen peaked between jointing to flowering stage. At maturity, nitrogen accumulation of the top fertilizer was higher than that of the base fertilizer. Plant nitrogen accumulation from soil under N150 was higher than that from nitrogen fertilizer, but with an opposite tendency under N225 and N300. With the increases of nitrogen application rate, the residual nitrogen in the 0-100 cm soil layer in the maturing stage significantly increased, while the residual nitrogen ratio in the 60-100 cm soil layer gradually increased.